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植物源单克隆抗体
植物源单克隆抗体
PART
pFolia™ β-actin Antibody, mAb, Mouse
Product Specific Information
Product is shipped on ice and should be stored at -20 °C upon receipt.
Target Background and Synonyms
Beta actin antibodies provide a specific and useful tool for studying the intracellular distribution of beta actin and the static and dynamic aspects of the cytoskeleton. Because it is ubiquitously expressed in all eukaryotic cells, beta actin is frequently used as a loading control.
| Product Details | |
| Size | 100 μL |
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host/lsotype | Mouse/IgG |
| Class | monoclonal |
| Type | Antibody |
| Form | Liquid |
| Conjugate | Unconjugated |
| Immunogen | β-actin |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 20 mM PBS, 150 mM NaCl, pH 7.4 |
| Storage condition | -20°C, avoiding freeze cycles |
| Catalog No. | M020101 |
| Applications | Tested Dilution |
| WB | 1:20000-1:100000 |
| IP | 0.5-4.0μg for IP and 1:5000-1:50000 for WB |
| IHC | 1:20-1:2000 |
| IF | 1:500-1:2000 |
Product Images For pFolia™ β-actin Antibody
- 1：HUVEC lysates,7.4μg
- 2：HUVEC lysates,1.5μg
- 3：HUVEC lysates,0.3μg
Comparative β-actin detection by Western blot was performed using pFolia™ β-actin antibody，M020101(A) and an anti- β-actin antibody product of a leading supplier (B) both at a concentration of 0.1 µg/mL. Both antibodies were used to probe the same HUVEC（ Human Umbilical Vein Endothelial Cells） lysates containing β-actin.
PART
pFolia™ DYKDDDDK Tag Antibody, mAb, Mouse
Product Specific Information
Product is shipped on ice and should be stored at -20 °C upon receipt.
roduct Specific Information
The DYKDDDDK has been used extensively as a general epitope tag in expression vectors. pFolia™ DYKDDDDK Tag Antibody recognizes the N-terminal, C-terminal or internal DYKDDDDK tagged (also known as the FLAG® tag sequence) fusion proteins.
| Product Details | |
| Size | 100 μL |
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host/lsotype | Mouse/IgG |
| Class | monoclonal |
| Type | Antibody |
| Form | Liquid |
| Conjugate | Unconjugated |
| Immunogen | DYKDDDDK |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 20 mM PBS, 150 mM NaCl, pH 7.4 |
| Storage condition | -20°C, avoiding freeze cycles |
| Catalog No. | M061201 |
| Applications | Tested Dilution |
| WB | 0.1-0.5 µg/mL |
| ELISA | 0.05-0.2 µg/mL |
Product Images For pFolia™ DYKDDDDK Tag Antibody
Western blot analysis of a FLAG-DsRed titration: Dereasing amount of recombinant FLAG-DsRed were separated on SDS-PAGE and blotted with pFolia™ DYKDDDDK Tag Antibody, mAb (left) or an anti- FLAG antibody product of a leading supplier (right) both at a concentration of 0.1 µg/mL.
PART
pFolia™ His Tag Antibody, mAb, Mouse
Product Specific Information
Product is shipped on ice and should be stored at -20 °C upon receipt.
Target Background and Synonyms
The histidine peptide is used as a tag on many recombinant proteins to facilitate purification.Further analysis of the protein can be achieved using an antibody specific to the His tag in techniques such as western blotting. The antibody recognizes the His-tag fused to targeted proteins.
| Product Details | |
| Size | 100 μL |
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host/lsotype | Mouse/IgG |
| Class | monoclonal |
| Type | Antibody |
| Form | Liquid |
| Conjugate | Unconjugated |
| Immunogen | Histidine |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 20 mM PBS, 150 mM NaCl, pH 7.4 |
| Storage condition | -20°C, avoiding freeze cycles |
| Catalog No. | M080901 |
| Applications | Tested Dilution |
| WB | 0.1-0.5 µg/mL |
| ELISA | 0.05-0.2 µg/mL |
Product Images For pFolia™ His Tag Antibody
Comparative His-tag detection by Western blot was performed using pFolia™ His tag antibody， M080901 (A) and an Anti-His antibody product of a leading supplier (B) both at a concentration of 0.1 µg/mL. Both antibodies were used to probe the same recombinant protein ：His-DsRed.
PART
pFolia™ GAPDH Antibody, mAb, Mouse
Product Specific Information
Product is shipped on ice and should be stored at -20 °C upon receipt.
Target Background and Synonyms
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. pFolia™ GAPDH antibody is raised against full length GAPDH of human origin. It can recognize the 36 KDa GAPDH protein in most cells/tissues.
| Product Details | |
| Size | 100 μL |
| Species Reactivity | human, mouse, rat, yeast, plant |
| Published Species | human, mouse, rat, tobacco |
| Host/lsotype | Mouse/IgG |
| Class | monoclonal |
| Type | Antibody |
| Form | Liquid |
| Conjugate | Unconjugated |
| Immunogen | N terminal region of human GAPDH |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 20 mM PBS, 150 mM NaCl, pH 7.4 |
| Storage condition | -20°C, avoiding freeze cycles |
| Catalog No. | M070101 |
| Applications | Tested Dilution |
| Western Blot | 0.002-0.02 μg/mL |
| Immunoprecipitation | 0.5-4.0 μg for cell lysate (3 mg) |
| Immunofluorescence | 0.5-5 µg/mL |
Product Images For pFolia™ GAPDH Antibody
Left panel: Whole plant tissue extract from tobacco, arabidopsis, rice and wheat were subjected to SDS- PAGE followed by Western Blot with M070101 (GAPDH Antibody) at dilution of 1: 10000. Right panel: Various lysates were subjected to SDS-PAGE followed by Western Blot with M070101 (GAPDH Antibody) at dilution of 1:50000.
